ABSTRACT
Purpose@#This study was to investigate the work experience of newly recruited male nurses during the COVID-19 pandemic. @*Methods@#With a phenomenological approach, this qualitative study was adopted semistructured interviews by phone or video calls. A total of 9 male nurses newly recruited for the COVID-19 wards in Chinese hospitals were interviewed for this study. And Colaizzi's method was applied for evaluation in the data analysis. @*Results@#Based on our findings, three themes were extracted. First, the newly recruited male nurses showed negative emotions at the beginning of COVID-19 epidemic, which was caused by changes in working conditions and content, but also prompted the nurses to change the way of coping with the crisis. Second, they gradually mastered the working skills and psychological training to cope with COVID-19 and developed a positive attitude toward life and a high sense of professional responsibility. Finally, we learned about their needs to respond to public health emergencies such as the COVID-19 pandemic. @*Conclusion@#COVID-19 is a disaster for all of humanity. The newly recruited male nurses are an important force in emergency rescue. Although they suffered from short-term negative emotions, they quickly adapted to the crisis. In order to better prepare for future emergencies, the disaster response capacity of newly recruited male nurses needs to be further improved. In addition, newly recruited male nurses have a strong demand for timely and personalized career development guidance.
ABSTRACT
Purpose@#This study was to investigate the work experience of newly recruited male nurses during the COVID-19 pandemic. @*Methods@#With a phenomenological approach, this qualitative study was adopted semistructured interviews by phone or video calls. A total of 9 male nurses newly recruited for the COVID-19 wards in Chinese hospitals were interviewed for this study. And Colaizzi's method was applied for evaluation in the data analysis. @*Results@#Based on our findings, three themes were extracted. First, the newly recruited male nurses showed negative emotions at the beginning of COVID-19 epidemic, which was caused by changes in working conditions and content, but also prompted the nurses to change the way of coping with the crisis. Second, they gradually mastered the working skills and psychological training to cope with COVID-19 and developed a positive attitude toward life and a high sense of professional responsibility. Finally, we learned about their needs to respond to public health emergencies such as the COVID-19 pandemic. @*Conclusion@#COVID-19 is a disaster for all of humanity. The newly recruited male nurses are an important force in emergency rescue. Although they suffered from short-term negative emotions, they quickly adapted to the crisis. In order to better prepare for future emergencies, the disaster response capacity of newly recruited male nurses needs to be further improved. In addition, newly recruited male nurses have a strong demand for timely and personalized career development guidance.
ABSTRACT
Objective To investigate the effects of rotavirus ( RV) on the expression and bioactiv-ity of Na+-H+ exchanger 3 ( NHE3 ) in Caco-2 cells and the possible regulatory mechanism. Methods Caco-2 cells expressing NHE3 were constructed and divided into four groups as follows: control ( CTL ) group, RV group, BAPTA-AM ( a Ca2+ chelator) group and BAPTA-AM+RV group. Na+-H+ exchanger ac-tivity and NHE3 expression on cell surface were determined using BCECF-AM and biotinylation assay, re-spectively. Expression of Cdc42 at protein level was measured by Western blot. Results Compared with the control group, RV infection significantly decreased the activity of NHE3 and its expression on cell surface. BATPA-AM antagonized the inhibitory effects on NHE3. Moreover, the expression of Cdc42 at protein level was increased following RV infection, which was also antagonized by BATPA-AM. Conclusions Intracellu-lar Ca2+-mediated Cdc42-dependent endocytosis pathway might be involved in regulating the expression and bioactivity of NHE3 during RV infection.
ABSTRACT
Interventions of expressive writing in cancer patients are mainly implemented in foreign countries. This paper reviews the proposal and development, implementation methods and mechanisms of expressive writing, and its effect on health outcomes of cancer patients, which indicates the population sensitivity and cultural sensitivity.In recent years,more and more attention has been paid to the intermediary variables, objective index and the analysis of the writing text in order to fully explain the mechanism and identify the social psychological needs of cancer patients at different stages. Future research can focus on sensitive populations, explore intermediary variables, incorporate objective indicators, optimize intervention methods, and explore the best intervention model for localization.
ABSTRACT
Objective To observe the effects and regulatory mechanism of rotavirus infection on the expression and bioactivity of Na+/H+exchanger 3 (NHE3) on Caco-2 cells. Methods A cell model of Caco-2 cells expressing NHE3 was constructed. Four groups were set up,which were control(CTL) group, rotavirus(RV) infection group, Cdc42 inhibitor (Pirl-1) group and Pirl-1+RV group. Bioactivity and ex-pression of NHE3 on the surface of Caco-2 cells were determined by BCECF-AM and biotinylation method, respectively. Expression of Cdc42 protein was measured by Western blot. Co-immunoprecipitation was per-formed to detect the interaction between NHE3 and Cdc42. Results Compared with the CTL group,RV in-fection significantly inhibited the bioactivity and expression of NHE3 on Caco-2 cells. These inhibitory effects were antagonized by Pirl-1. Moreover,RV infection enhanced the expression of Cdc42 protein and promoted the interaction between NHE3 and Cdc42, which were also antagonized by Pirl-1. Conclusion RV infec-tion might regulate the expression and bioactivity of NHE3 through Cdc42-dependent endocytosis pathway.
ABSTRACT
Objective To observe the effect of rotavirus (RV) infection on expression level and bioactivity of Na+-H+ exchanger 3 (NHE3) in Caco-2 cells. Methods Model of NHE3-expressing Caco-2 cells was constructed and studied in terms of intervention: control, RV, clathrin antagonist chlorpromazine (CPZ), and CPZ + RV. NHE3 activity and NHE3 protein amount on cell surface were determined by BCECF-AM and biotinylation, respectively. Expression level of clathrin was assayed by Western blot. Results Compared with control group, NHE3 activity and NHE3 surface protein level significantly decreased when the cells were treated with RV. These effects could not be completely cancelled by clathrin antagonist CPZ. Moreover, RV treatment could increase cellular protein level of clathrin, which was cancelled by CPZ. Conclusions The effect of RV infection on NHE3 expression level and biological activity may be related to clathrin-dependent endocytosis pathway, and may be also affected by other endocytosis pathways.
ABSTRACT
Objective To evaluate the effects of mindfulness-based stress reduction(MBSR) on fatigue and sleep quality for breast cancer patients undergoing chemotherapy. Methods Totally 96 patients with breast cancer under-going chemotherapy were assigned into the intervention group and the control group with 48 cases in each group from a tertiary hospital in Zhengzhou city. Both groups received routine care,and the intervention group also received 8 weeks of MBSR. The Revised Piper Fatigue Scale(PFS-R) and Pittsburgh Sleep Quality Index(PSQI) were used to assess fatigue and sleep quality of two groups during chemotherapy before the intervention(T1),after the interven-tion(T2) and 3 months after the intervention (T3). Results There were significant differences in sensory,behav-ioral,emotional and total fatigue scores between the two groups(P<0.05);the time effect on fatigue of the various dimensions scores and total fatigue scores was significant(P<0.05);sensory aspects,emotional aspects,behavioral aspects,and total fatigue scores were interfered between intervention and time factors(P<0.05). There were signifi-cant differences in sleep quality,fall sleep time,sleep time,sleep efficiency and sleep scores between the two groups(P<0.05);the time effect on sleep quality,sleep time, fall sleep time was significant(P<0.05);there were interac-tion effects between intervention and time on sleep quality,sleep time,sleep disturbance and sleep total score(P<0.05). Conclusion MBSR can effectively relieve fatigue and improve sleep quality for breast cancer patients undergoing chemotherapy.
ABSTRACT
Objective To evaluate the application and efficacy of 6S quality management methods in drug clinical trials of oncology department.Methods By using 6S (Seiri,Seiton,Seiso,Seiketsu,Shitsuke,Safety) quality management methods,quality about mastering level of good clinical practice (GCP) knowledge,sample collection and drug management etc.were controlled,and efficacy after the quality control was evaluated.Results After implemention of 6S quality management,rate of achieving GCP certificate was increased to 77.80％,accuracy rate of sample collection and accuracy rate of medicine preparation were increased to 100.00％,and the rate of relearning study protocol was increased to 100.00％,and subjects' satisfaction was improved significantly.Conclusion The implementation of 6S quality management methods could effectively enhance the quality of drug clinical trials in oncology department.
ABSTRACT
Objective To translate the English version of Safety Attitudes Questionnaire (SAQ) into Chinese,and to test the reliability and validity of the Chinese version of SAQ. Methods The SAQ was developed into Chinese version through translation, back-translation and cross-culture adaption. The reliability and validity of the Chinese version of SAQ were tested in 823 first-line nurse managers in Henan. Results The scale content validity index was 0.90 of the Chinese SAQ, and the item content validity index ranged from 0.83 to 0.96. The correlations between the items and the full scale, the dimensions and the full scale separately ranged from 0.32 to 0.64 and 0.50 to 0.81 (P<0.01). The confirmatory factor analysis resulted in a good model fit for the six dimensions and the full construct, the goodness-of-fit index was 0.96, Tucker-Lewis index was 0.93, comparative fit index was 0.95, and root mean square error of approximation was 0.06. The Cronbach alpha coefficient for the scale was 0.913 and for the six dimensions were 0.565, 0.655, 0.825, 0.804, 0.880, 0.725 separately, the test-retest reliability coefficient was 0.934. Conclusions The Chinese version of SAQ is a valid and reliable tool to assess the safety culture of first-line nurse managers in Chinese hospitals.
ABSTRACT
Objective To get a knowledge of the current status of international research on Ebola virus and Ebola virus disease (EVD).Methods All the articles about EVD from PubMed database were retrieved and analyzed.After core subject headings had been identified by BICOMB,the co-occurrence matrix was established.Cluster analysis about co-occurrence matrix was performed by SPSS 19.0.The visualized network image was drawn by Ucinet 6.0.Results A total of 1 384 literatures were involved in the analysis,with the earliest one published in 1979.The number of literatures was increasing along with time and disease outbreaks.Totally 1 213 (87.6％) literatures were published by Europe and U.S.There were 27 core subjects and they were clustered into 3 research areas from 1979 to 2014,including Ebola virus genome structure and viral proteins,especially the envelope protein; immunology of Ebola virus and animal models; epidemiology,diagnosis and treatment of Ebola hemorrhagic fever.The visualized social network reflected that the prevention of EVD,the genome and immunology of Ebola were the major concerns.Conclusion It has been 35 years since the literatures on Ebola virus and EVD emerged.The United States and the United Kingdom are the top two countries in this field.The present studies have laid a crucial foundations for developing effective vaccines and diagnostic reagents.